Description:
X-α-Gal;
5-Bromo-4-chloro-3-indoxyl-α-D-galactopyranoside;
X-α-D-Galactoside

MOLECULAR BIOLOGY GRADE

C₁₄H₁₅BrCNO₆

MW: 408.64 g/mol

Chromogenic substrate for α-galactosidase yielding a blue precipitate. X-α-Gal is used for detecting two-hybrid interactions directly on the culture media. This substrate eliminates the need for β-galactosidase liquid and filter-lift assays in two-hybrid analysis.
Used for species differentiation within the family Enterobacteriaceae and differentiation of Bifidobacteria species from Lactobacillus species.

Store at -15°C. Protect from light.

References:

1. Aho S., Arffman A., Pummi T., Uitto J., A novel reporter gene MEL1 for the yeast two-hybrid system. Anal Biochem. 1997. 253, 270-2.

2. Chevalier P., et al., X-α-Gal-base medium for simultaneous enumeration of bifidobacteria and lactic acid bacteria in milk. J. Microbiol. Methods. 1991. 13, 75.

3. Gossrau R., Lojda Z., Histochemical detection of α-D-galactosidase with 5-Br-4-Cl-3-indoxyl α-D-galactoside. Acta Histochem. 1989. 85, 213-22.

4. Perry J.D., Ford M., Taylor J., Jones A.L., Freeman R., Gould F.K., ABC medium, a new chromogenic agar for selective isolation of Salmonella spp. J. Clin. Microbiol. 1999. 37, 766-8.