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Phospho-p90RSK (Thr359/Ser363) Antibody
产品编号 9344
供应商 CST
规格 100ul
目录价 3487.00现货7折促销

Applications Reactivity Sensitivity MW (kDa) Source
W IP IHC-P H M R Mk Endogenous 90 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Phospho-p90RSK (Thr359/Ser363) Antibody detects endogenous levels of p90RSK only when phosphorylated at Thr359/Ser363. This antibody does not recognize p90RSK phosphorylated at other sites. It shows some cross-reactivity with RSK3 when phosphorylated at the homologous sites, but no cross-reactivity with phospho-RSK2 or -MSK1.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Thr359/Ser363 of rat RSK1. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

 

Western blot analysis of extracts from HeLa and C6 cells, untreated or TPA-treated (200 nM), using Phospho-p90RSK (Thr359/Ser363) Antibody (upper) or control RSK1/RSK2/RSK3 Rabbit mAb #9355 (lower).

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing nuclear localization using Phospho-p90RSK (Thr359/Ser363) Antibody.

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Phospho-p90RSK (Thr359/Ser363) Antibody.


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