|W IP IHC-P||H M R Mk||Endogenous||90||Rabbit|
Applications Key: W=Western Blotting IP=Immunoprecipitation IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Phospho-p90RSK (Thr359/Ser363) Antibody detects endogenous levels of p90RSK only when phosphorylated at Thr359/Ser363. This antibody does not recognize p90RSK phosphorylated at other sites. It shows some cross-reactivity with RSK3 when phosphorylated at the homologous sites, but no cross-reactivity with phospho-RSK2 or -MSK1.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Thr359/Ser363 of rat RSK1. Antibodies are purified by protein A and peptide affinity chromatography.
Western blot analysis of extracts from HeLa and C6 cells, untreated or TPA-treated (200 nM), using Phospho-p90RSK (Thr359/Ser363) Antibody (upper) or control RSK1/RSK2/RSK3 Rabbit mAb #9355 (lower).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing nuclear localization using Phospho-p90RSK (Thr359/Ser363) Antibody.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Phospho-p90RSK (Thr359/Ser363) Antibody.