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组蛋白去加急酶(H3-K4)活性/抑制分析试剂盒

【产品中文名称】

 组蛋白去加急酶(H3-K4)活性/抑制分析试剂盒

【产品产地】

 Epigentek

 English Name

 EpiQuik Histone Demethylase (H3-K4 Specific) Activity/Inhibition Assay Kit

产品货号

 P-3074

 产品 价格/规格

 48/4608元,96/7808

 【产品详细说明】

    

Product Overview


The
EpiQuik™ Histone Demethylase (H3-K4 Specific) Activity/Inhibition Assay Kit is a convenient set of tools that allows the experimenter to measure activity/inhibition of H3-K4 specific histone demethylases using cell/ tissue extracts. The kit is ready-to-use and provides all the essential components needed for measuring histone demethylase (H3-K4 Specific) activity/inhibition from a broad range of species including mammalian cells/tissues, plants, and bacteria.

WHY CHOOSE THE EPIQUIK™ HISTONE DEMETHYLASE (H3-K4 SPECIFIC) ACTIVITY/INHIBITION ASSAY KIT?

  • Fast procedure, which can be finished within 3 hours.
  • Innovative fluorescent assay with no use of radioactivity, extraction, and chromatography.
  • Direct measurement of HDM (H3-K4 specific) activity and inhibition and not interfered by thiol-containing chemicals such as DTT, GSH and 2-mercaptoethanol.
  • Strip microplate format makes the assay flexible: manual or high throughput analysis.
  • Simple, reliable, and consistent assay conditions.

Principle & Procedure


The EpiQuik™ Histone Demethylase (H3-K4 specific) Activity/Inhibition Assay Kit is designed for measuring total histone demetylase (H3-K4 Specific) activity/inhibition. In the assay with this kit, the unique di-methylated histone H3-K4 substrate is stably captured on the strip wells. Active HDMs bind to and demethylate histone H3-K4 substrate. The remaining un-demethylated substrate can be recognized with high affinity anti-methylated histone H3-K4 antibody. The ratio or amount of the un-demethylated histone, which is inversely proportional to HDM enzyme activity, can then be fluorimetrically quantified.

SCHEMATIC PROCEDURE:

Product Components


HD1 (10x wash buffer)
HD2 (HDM assay buffer)
HD3 (HDM substrate)*
HD4 (HDM standard, 20 µg/ml)*
HD5 (capture antibody 100 µg/ml)*
HD6 (detection antibody 200 µg/ml)*
HD7 (fluoro-developer)*
HD8 (fluoro enhancer)*
HD9 (fluoro-dilution)
8 well assay strip (with frame)
User guide


* For maximum recovery of the products, centrifuge the original vial after thawing prior to opening the cap.

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.


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