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Product Overview
The EpiQuik™ Acetyl-Histone H3 ChIP Kit is a convenient package of tools that allows the experimenter to investigate interactions of histone H3 acetylation and DNA in vivo efficiently. The entire procedure can be completed within 5 hours and produces far superior results than any competitor kits. The EpiQuik™ Acetyl-Histone H3 ChIP kit is suitable for combining the specificity of immunoprecipitation with qualitative and quantitative PCR, MS-PCR, DNA sequencing, and southern blot as well as DNA microarray.
WHY CHOOSE THE EPIQUIK™ ACETYL-HISTONE H3 ChIP KIT?
- The fastest procedure available, which can be finished within 5 hours.
- Strip microwell format makes the assay flexible: manual or high throughput.
- Columns for DNA purification are included: save time and reduce labor.
- Compatible with all DNA amplification-based approaches.
- Simple, reliable, and consistent assay conditions.
Principle & Procedure
The EpiQuik™ Acetyl-Histone H3 ChIP kit contains all reagents required for carrying out a successful chromatin immunoprecipitation for acetyl-histone H3 from mammalian cells. Particularly, this kit includes a ChIP-grade acetyl-histone H3 antibody and a negative control normal mouse IgG. Chromatin in the cells is extracted, sheared and added into the microwell immobilized with the antibody. Acetyl-histone H3 protein-DNA complex is captured by the antibody and DNA is released, and purified through the specifically designed Fast-Spin Column. Eluted DNA can be used for various down-stream applications.
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SCHEMATIC PROCEDURE: |
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Product Components
CP1 (wash buffer)
CP2 (antibody buffer)
CP3A (pre-lysis buffer)
CP3B (lysis buffer)
CP4 (CHIP dilution buffer)
CP5 (DNA release buffer)
CP6 (reverse buffer)
CP7 (binding buffer)
CP8 (elution buffer)
Protease inhibitor cocktail (100x)*
Proteinase K (10 mg/ml)*
Normal mouse IgG (0.5 mg/ml)*
Anti-acetyl-histone H3 (1 mg/ml)*
8-well assay strips (with frame)
8-well strip caps
F-spin column
F-collection tube
User guide
* Spin the solution down to the bottom before use.
User Guide & MSDS
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[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name. |
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