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通用蛋白-DNA结合分析试剂盒(Fluorometric)

【产品中文名称】

 通用蛋白-DNA结合分析试剂盒(Fluorometric)

【产品产地】

 Epigentek

 English Name

 EpiQuik General Protein-DNA Binding Assay Kit (Fluorometric)

产品货号

 P-2005

 产品 价格/规格

 96/5248

 【产品详细说明】

    

Product Overview


The
EpiQuik™ General Protein-DNA Binding Assay Kit (Fluorometric) (96 assays) is a convenient package of tools that allows the experimenter to investigate protein-DNA interaction in vitro efficiently. The entire procedure can be completed within 3 hours and produces far superior results than any competitor kits. The EpiQuik™ General Protein-DNA Binding Assay Kits are suitable for analyzing protein-DNA interactions in vitro, specifically for detecting transcription factor activation using mammalian tissue and cell extracts.

WHY CHOOSE THE EPIQUIK™ GENERAL PROTEIN-DNA BINDING ASSAY KIT (FLUOROMETRIC)(96 ASSAYS)?

  • The fastest procedure available: a complete assay can be finished within 3 hours.
  • Strip microplate format makes the assay flexible: manual or high throughput analysis.
  • Fluorometrically qualify or quantify protein activation and use radioactive-free materials: safer to handle.
  • No requirement for coating antibodies, or for using secondary antibodies, or for labeling oligo-nucleotides: save time and reduce labor.
  • Simple, reliable, and consistent assay conditions.

Principle & Procedure


The EpiQuik™ General Protein-DNA Binding Assay Kit (Fluorometric) is designed for measuring the transcription factor (TF) of DNA binding activity in nuclear extracts. In an assay with the use of this kit, target TF in nuclear extract is captured by high affinity antibody onto assay microwell. A double strand oligonucleotide (oligo) containing DNA binding consensus sequence for the target TF is added into the microwell and bound to active TF in the binding assay buffer. The bound oligo is then fluorometrically detected.

SCHEMATIC PROCEDURE:

Product Components


PF1 (10x wash buffer)
PF2 (antibody buffer)
PF3 (extract cleaner)*
PF4 (assay buffer)
PF5 (Oligo release solution)
PF6 (fluoro developer)*
DTT (500x)*
96-well assay plate
8-well assay strips (with frame)
User guide

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

[Material Safety Data Sheet]


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