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通用蛋白-DNA结合分析试剂盒(Colorimetric)

【产品中文名称】

 通用蛋白-DNA结合分析试剂盒(Colorimetric)

【产品产地】

 Epigentek

 English Name

 EpiQuik General Protein-DNA Binding Assay Kit (Colorimetric)

产品货号

 P-2004

 产品 价格/规格

 96/5248

 【产品详细说明】

    

Product Overview


The
EpiQuik™ General Protein-DNA Binding Assay Kit (Colorimetric) (96 assays) is a convenient package of tools that allows the experimenter to investigate protein-DNA interaction in vitro efficiently. The entire procedure can be completed within 3 hours and produces far superior results than any competitor kits. The EpiQuik™ General Protein-DNA Binding Assay Kits are suitable for analyzing protein-DNA interactions in vitro, specifically for detecting transcription factor activation using mammalian tissue and cell extracts.

WHY CHOOSE THE EPIQUIK™ GENERAL PROTEIN-DNA BINDING ASSAY KIT (COLORIMETRIC)(96 ASSAYS)?

  • The fastest procedure available: a complete assay can be finished within 3 hours.
  • Strip microplate format makes the assay flexible: manual or high throughput analysis.
  • Colorimetrically quantifies protein activation and uses radioactive-free materials: safer to handle.
  • Simple, reliable, and consistent assay conditions.

Principle & Procedure


The EpiQuik™ General Protein-DNA Binding Assay Kit (Colorimetric) is designed for measuring the transcription factor of DNA binding activity in nuclear extracts. In an assay with the use of this kit, a biotin-labeled double stranded oligonucleotide (oligo) containing DNA binding consensus sequence for the target transcription factor is incubated with nuclear extract in the binding assay buffer. Active form of transcription factor in the nuclear extract binds to its consensus sequence. Oligo-protein complex is then captured onto the assay microwell. The target protein can be recognized by high affinity antibody and colorimetrically measured through detection antibody-color development reagent reaction system.

SCHEMATIC PROCEDURE:

Product Components


PD1 (10x wash buffer)
PD2 (assay binding buffer)
PD3 (antibody dilution buffer)
PD4 (developing solution)
PD5 (stop solution)
8-well assay strips (with frame)
User guide

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

[Material Safety Data Sheet]


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