您所在的位置:首页 > 组织染色质免疫沉淀(ChIP)试剂盒
组织染色质免疫沉淀(ChIP)试剂盒

【产品中文名称】

 组织染色质免疫沉淀(ChIP)试剂盒

【产品产地】

 Epigentek

 English Name

 EpiQuik Tissue Chromatin Immunoprecipitation (ChIP) Kit

产品货号

 P-2003

 产品 价格/规格

 24/3488,48/5584元,96/8624

 【产品详细说明】

    

Product Overview


The
EpiQuik™ Tissue Chromatin Immunoprecipitation (ChIP) Kit is a convenient package of tools that allows the experimenter to perform chromatin immunoprecipitation (ChIP) from mammalian tissues at extraordinarily rapid speeds and consistency, superior to all other current ChIP methods available. The kit is ready-to-use and provides all the essential components needed to carry out a successful ChIP experiment on tissues. The EpiQuik™ ChIP kits are suitable for combining the specificity of immunoprecipitation with qualitative and quantitative PCR, MS-PCR, DNA sequencing, and southern blot as well as DNA microarray.

WHY CHOOSE THE EPIQUIK™ TISSUE CHROMATIN IMMUNOPRECIPITATION CHIP KIT?

  • Straightforwardly put: It is the truly number one fastest procedure available. The entire procedure can be completed within an amazingly short 5 hour period with superb results.
  • The ChIP procedure has been drastically simplified -- extremely short and easy to follow.
  • Strip microplate format makes the assay flexible: manual or high throughput.
  • Columns for DNA purification are included: save tremendous amounts of time and reduce unnecessary physical labor.
  • Compatible with all DNA amplification-based approaches.
  • Achieves very reliable and consistent assay conditions.

Principle & Procedure


The EpiQuik™ Tissue Chromatin Immunoprecipitation kit contains all reagents required for carrying out a successful chromatin immunoprecipitation from mammalian tissues. Particularly, this kit includes a positive control antibody (RNA polymerase II), a negative control normal mouse IgG, and GAPDH primers that can be used as a positive control to demonstrate the efficacy of the kit reagents and protocol. RNA polymerase II is considered to be enriched in the GAPDH gene promoter that is expected to be undergoing transcription in most growing mammalian cells and can be immunoprecipitated by RNA polymerase II but not by normal mouse IgG.

In this ChIP, cells are cross-linked with formaldehyde and chromatin is extracted. The chromatin is then sheared and added into the microwell immobilized with affinity antibodies. Cross-linked DNA is released from antibody-captured protein-DNA complex, reversed and purified through the specifically designed Fast-Spin Column. Eluted DNA can be used for various down-stream applications.

SCHEMATIC PROCEDURE:

COMPARATIVE OVERVIEW:

Product Components


CP1 (wash buffer)
CP2 (antibody buffer)
CP3 (lysis buffer)
CP4 (CHIP dilution buffer)
CP5 (DNA release buffer)
CP6 (reverse buffer)
CP7 (binding buffer)
CP8 (elution buffer)
Homogenizing buffer
Protease inhibitor cocktail (100x)*
Normal mouse IgG (1 mg/ml)*
Anti-RNA polymerase II (1mg/ml)*
Proteinase K (10 mg/ml)*
Control primer (GAPDH)
Forward (20 µM)*
Reverse (20 µM)*
8-well assay strips (with frame)
8-well strip caps
F-spin column
F-collection tube
User guide

* Spin the solution down to the bottom before use.

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

[Material Safety Data Sheet]

References & Citations


Fuchikami, Manabu E. et al. (Jun 2008). Single immobilization stress differentially alters the expression profile of transcripts of the brain-derived neurotrophic factor (BDNF) gene and histone acetylation at its promoters in the rat hippocampus. Int J Neuropsychopharmacol Epub ahead of print PubMed Abstract

O'Connor JC. et al. (May 2008). A novel antioxidant function for the tumour suppressor gene p53 in the retinal ganglion cell. Invest Ophthalmol Vis Sci Epub ahead of print PubMed Abstract

Aoyama, Tomoki et al. (Jan 2008). Cell-specific epigenetic regulation of ChM-I gene expression: Crosstalk between DNA methylation and histone acetylation. Biochem Biophys Res Commun. 365(1): 124-30. PubMed Abstract

Johnson, Carrie E. et al. (Dec 2007). Differential Apaf-1 levels allow cytochrome c to induce apoptosis in brain tumors but not in normal neural tissues. Proceedings of the National Academy of Sciences of the USA 104(52): 20820-5. PubMed Abstract


pyright 2004-2009 www.bioso.cn 金佰利娱乐有限公司版权所有 订货热线:4008811876
电话:010-62917543 87175666 87175777 传真:010-62913373 邮箱:bioso@yahoo.cn 技术:biosou@yahoo.cn 网站统计