The EpiQuik™ DNA Methyltransferase 1 Activity/Inhibitor Screening Assay Kit is a convenient set of tools that allows the experimenter to selectively screen Dnmt1 activity/inhibitors. The selective inhibition of Dnmt1 may lead to demethylation and expression of the silenced tumor suppressor genes. Thus the selective Dnmt1 inhibitors could be new addition to cancer therapeutic agents. The kit is ready-to-use and provides all the essential components needed to carry out a successful Dnmt1 activity/inhibitor screening experiment without the need for radioactivity or any special equipment.
WHY CHOOSE THE EPIQUIK™ DNA METHYLTRANSFERASE ACTIVITY/INHIBITOR SCREENING ASSAY KIT?
- Very rapid procedure, which can be completed within 3 hours.
- Safe and innovative colorimetric assay without radioactivity, extraction, and chromatography.
- Strip microplate format makes the assay flexible: manual or high throughput analysis.
- Extremely simple, reliable, and consistent assay conditions.
Principle & Procedure
The EpiQuik™ DNA Methyltransferase 1 Activity/Inhibitor Screening Assay Kit is designed for screening Dnmt 1 inhibitors. In an assay with this kit, the unique cytosine-rich DNA substrate is stably coated on the strip wells. These wells are specifically treated to have a high DNA absorption ability. Dnmt 1 enzymes transfer methyl group to cytosine from Adomet to methylate DNA substrate. The methylated DNA can be recognized with anti-5-methylcytosine antibody. The ratio or amount of methylated DNA, which is proportional to enzyme activity, can then be colorimetrically quantified through an ELISA-like reaction.
MO1 (10x wash buffer)
MO2 (Dnmt assay buffer)
MO4 (active Dnmt1, 200 µg/ml)*
MO5 (capture antibody )*
MO6 (detection antibody )*
MO7 (developing solution)
MO8 (stop solution)
8 well substrate-coated strip (with frame)
* For maximum recovery of the products, centrifuge the original vial after thawing prior to opening the cap.
User Guide & MSDS
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing firstname.lastname@example.org along with your contact information and institution name.
References & Citations
Liu, Zhongfa et al. (Feb 2009). Modulation of DNA Methylation by a Sesquiterpene Lactone Parthenolide. J Pharmacol Exp Ther. Epub Ahead of Print. PubMed Abstract