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Dnmt1 分析试剂盒

【产品中文名称】

 Dnmt1 分析试剂盒

【产品产地】

 Epigentek

 English Name

 EpiQuik Dnmt1 Assay Kit

产品货号

 P-3011

 产品 价格/规格

 24/3488元,48/5744元,96/7984

 【产品详细说明】

    

Product Overview


The
EpiQuik™ Dnmt1 Assay Kit is a convenient package of tools that allows the experimenter to measure the amount of Dnmt1 at tremendously fast speeds and consistency, superior and safer than all other current methods. The kit is ready-to-use and provides all the essential components needed to carry out a successful Dnmt1 assay experiment without the need for electrophoresis and transfer process. The EpiQuik™ Dnmt1 Assay Kit is suitable for measuring Dnmt amounts quantitatively from fresh tissue and cultured cells of human and mouse.

WHY CHOOSE THE EPIQUIK™ DNMT1 ASSAY KIT?

  • The fastest procedure, which can be finished within 3 hours.
  • Innovative colorimetric assay to quantitatively measure Dnmt1 amount with no need of electrophoresis.
  • Strip microplate format makes the assay flexible: manual or high throughput analysis.
  • Simple, reliable, and consistent assay conditions.

Principle & Procedure


The EpiQuik™ Dnmt1 Assay Kit is designed for measuring total Dnmt1 amount from tissues or cells. In an assay with this kit, the unique Dnmt affinity substrate is stably coated on the strip well. The sample is added into the well and Dnmt1 contained in the sample binds to the substrate. The bound Dnmt1 can be recognized with specific Dnmt1 antibody and colorimetrically quantified through an ELISA-like reaction. The amount of Dnmt1 is proportional to the intensity of color development.

SCHEMATIC PROCEDURE:

Product Components


D1 (10x wash buffer)
D2 (assay buffer)
D3 (Dnmt1 standard, 20 µg/ml)*
D4 (affinity antibody 100 µg/ml)*
D5 (detection antibody 200 µg/ml)*
D6 (developing solution)
D7 (stop solution)
8 well assay strips (with frame)
User guide

* For maximum recovery of the products, centrifuge the original vial after thawing prior to opening the cap.

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

[Material Safety Data Sheet]

References & Citations


Hervouet, Eric et al. (November 2008). Tumor induction by disruption of the Dnmt1, PCNA and UHRF1 interactions. Nature Precedings. Full PDF Article

Roll, J Devon et al. (Jan 2008). DNMT3b overexpression contributes to a hypermethylator phenotype in human breast cancer cell lines. Mol Cancer
7: 15. Full PDF Article


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