您所在的位置:首页 > DNA去甲基化酶活性/抑制分析试剂盒
DNA去甲基化酶活性/抑制分析试剂盒

【产品中文名称】

 DNA去甲基化酶活性/抑制分析试剂盒

【产品产地】

 Epigentek

 English Name

 EpiQuik DNA Demethylase Activity/Inhibition Assay

产品货号

 P-3019

 产品 价格/规格

 48/5088元,96/7984

 【产品详细说明】

    

Product Overview


The
EpiQuik™ DNA Demethylase Activity/Inhibition Assay Kit is suitable for measuring DNA demethylase activity/inhibition using purified enzyme or protein extracts from the cultured cells and tissues.

WHY CHOOSE THE EPIQUIK™ DNA DEMETHYLASE ACTIVITY/INHIBITION ASSAY KIT?

  • Quick and efficient, which can be finished within 5 hours.
  • Innovative colorimetric assay with no need for radioactivity, electrophoresis, and chromatography.
  • Strip microwell format makes the assay flexible: manual or high throughput.
  • Simple, reliable, and consistent assay conditions.

Principle & Procedure


The EpiQuik™ DNA Demethylase Activity/Inhibition Assay Kit is designed for measuring total DNA demethylase activity/inhibition. In an assay with this kit, the unique methylated DNA substrate is stably captured on the strip wells. Active DNA demethylases bind to and demethylate DNA substrate. The methylated DNA can be recognized with high affinity 5-methycytosine antibody. The ratio or amount of methylated DNA, which is inversely proportional to enzyme activity, can be then colorimetrically quantified through an ELISA-like reaction.

SCHEMATIC PROCEDURE:

Product Components


DD1 (10x wash buffer)
DD2 (demethylase assay buffer)
DD3 (demethylase substrate)*
DD4 (demethylation standard, 10 µg/ml)*
DD5 (capture antibody, 1000 µg/ml)*
DD6 (detecting antibody, 200 µg/ml)*
DD7 (developing solution)
DD8 (stop solution)
8 well assay strips (with frame)
User guide

* For maximum recovery of the products, centrifuge the original vial after thawing prior to opening the cap.

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

[Material Safety Data Sheet]


pyright 2004-2009 www.bioso.cn 金佰利娱乐有限公司版权所有 订货热线:4008811876
电话:010-62917543 87175666 87175777 传真:010-62913373 邮箱:bioso@yahoo.cn 技术:biosou@yahoo.cn 网站统计