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组织甲基化DNA免疫沉淀试剂盒

【产品中文名称】

 组织甲基化DNA免疫沉淀试剂盒

【产品产地】

 Epigentek

 English Name

 EpiQuik Tissue Methylated DNA Immunoprecipitation

产品货号

 P-2020

 产品 价格/规格

 24/4768,48/7984

 【产品详细说明】

    

Product Overview


The
EpiQuik™ Tissue Methylated DNA Immunoprecipitation (MDIP) Kit is a complete set of essential components which uses a proprietary and unique procedure/composition to enrich methylated DNA from various mammalian tissues. In the assay, an antibody specific to methyl cytosine is used to immunoprecipitate methylated genomic DNA. The immunoprecipitated methylated fractions can be then used for a standard DNA detection. The kit is suitable for combining the specificity of methylated DNA immunoprecipitation with qualitative and quantitative PCR, and southern blot as well as DNA microarray.

WHY CHOOSE THE EPIQUIK™ TISSUE MDIP KIT?

  • Directly immunoprecipitate methylated fractions of DNA from tissue lysates.
  • Highly efficient enrichment of methylated DNA: > 95%
  • The fastest procedure available, which can be finished within 3 and a half hours.
  • Strip microwell format makes the assay flexible: manual or high throughput.
  • Columns for DNA purification are included: save time and reduce labor.
  • Compatible with all DNA amplification-based approaches.
  • Simple, reliable, and consistent assay conditions.

Principle & Procedure


The EpiQuik™ Tissue Methylated DNA Immunopricipitation (MDIP) Kit contains all reagents required for carrying out a successful methylated DNA immunoprecipitation directly from mammalian tissues. Particularly, this kit includes a ChIP-grade 5-methylcytosine antibody and a negative control normal mouse IgG. DNA in the cells is extracted, sheared and added into the microwell immobilized with the antibody. DNA is released from the antibody-DNA complex, and purified through the specifically designed Fast-Spin Column. Eluted DNA can be used for various down-stream applications.

SCHEMATIC PROCEDURE:

Product Components


CP1 (wash buffer)
CP2 (antibody buffer)
CP3 (lysis buffer)
CP4 (CHIP dilution buffer)
CP5 (DNA release buffer)
CP6 (reverse buffer)
CP7 (binding buffer)
CP8 (elution buffer)
Homogenizing buffer
Normal mouse IgG (1 mg/ml)*
Anti-5-methylcytosine (1mg/ml)*
Proteinase K (10 mg/ml)*
8-well assay strips (with frame)
8-well strip caps
F-spin column
F-collection tube
User guide

* Spin the solution down to the bottom before use.

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

[Material Safety Data Sheet]


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