The Methylamp™ Methylated DNA Capture Kit is a complete set of essential components which enables the experimenter to use proprietary and unique procedure/compositions to enrich methylated DNA. In the assay, an antibody specific to methyl cytosine is used to capture methylated genomic DNA. The enriched methylated fractions can be then used for a standard DNA detection. The entire procedure can be completed within 3 hours and produces far superior results than any competitor kits. The Methylamp™ Methylated DNA Capture Kit is suitable for combining the specificity of enriched methylated DNA with qualitative and quantitative PCR, and southern blot as well as DNA microarray.
WHY CHOOSE THE METHYLAMP™ METHYLATED DNA CAPTURE KIT?
- Highly efficient enrichment of methylated DNA: > 98%.
- The fastest procedure available, which can be finished within 3 hours.
- Strip microplate format makes the assay flexible: manual or high throughput.
- Columns for DNA purification are included: save time and reduce labor.
- Compatible with all DNA amplification-based approaches.
- Simple, reliable, and consistent assay conditions.
Principle & Procedure
The Methylamp™ Methylated DNA Capture Kit contains all reagents required for carrying out a successful capture of methylated DNA from a DNA sample. Particularly, this kit includes a ChIP-grade 5-methylcytosine antibody and a negative control normal mouse IgG. DNA is sheared, added into the microwell and captured by the antibody. DNA is released from the antibody-captured methylated DNA complex, and purified through the specifically designed Fast-Spin Column. Eluted DNA can be used for various down-stream applications.
MC1 (antibody buffer)
MC2 (Reaction buffer)
MC3 (wash buffer)
MC4 (DNA release buffer)
MC5 (binding buffer)
MC6 (elution buffer)
Normal mouse IgG (1 mg/ml)*
Proteinase K (10 mg/ml)*
8-well assay strips (with frame)
8-well strip caps
* Spin the solution down to the bottom before use.
Frequently Asked Q's
What is the difference between the Methylamp Methylated DNA Capture Kit (#P-1015) and the EpiQuik Methylated DNA Immunoprecipitation Kit (#P-2019)?
The Methylamp Methylated DNA Capture Kit is for purified DNA as the starting materials while the EpiQuik Methylated DNA Immunoprecipitation Kit is for nuclear extract as the starting materials.
User Guide & MSDS
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing email@example.com along with your contact information and institution name.