The Methylamp™ Global DNA Methylation Quantification Kit is a complete set of essential components which enables the experimenter to quantify global DNA methylation through an ELISA-like reaction using Epigentek's uniquely simplified and streamlined procedure. The entire procedure can be completed within only 4 hours in either manual or high throughput analysis. The Methylamp™ Global DNA Methylation Quantification Kit is suitable for detecting global DNA methylation status using genomic DNA isolated from variety of cultured cells, fresh and frozen tissue, paraffin-embedded tissue, plasma/serum sample, and body fluid sample, etc.
WHY CHOOSE THE METHYLAMP™ GLOBAL DNA METHYLATION QUANTIFICATION KIT?
- Very rapid procedure, which can be finished within 4 hours.
- Colorimetric quantification without radioactivity, extraction, and chromatography.
- Strip microplate format makes the assay flexible: manual or high throughput analysis.
- Simple, reliable, and consistent assay conditions.
Principle & Procedure
The Methylamp™ Global DNA Methylation Quantification Kit contains all reagents required for quantification of global DNA methylation. In this assay, DNA is immobilized to the strip well specifically treated to have high affinity to the DNA. The methylated fraction of DNA can be recognized by 5-methylcytosine antibody and quantified through an ELISA-like reaction. The amount of methylated DNA is proportional to the OD intensity.
Quantification of Methylated DNA.
GM1 (10x wash buffer)
GM2 (DNA binding solution)
GM3 (methylated DNA control,100 µg/ml)*
GM4 (block solution)
GM5 (capture antibody, 1 mg/ml)*
GM6 (detecting antibody, 100 µg/ml)*
GM7 (developing solution)
GM8 (stop solution)
8 well assay strips (with frame)
* Spin the solution down to the bottom before use.
User Guide & MSDS
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing firstname.lastname@example.org along with your contact information and institution name.
References & Citations
Hervouet, Eric et al. (November 2008). Tumor induction by disruption of the Dnmt1, PCNA and UHRF1 interactions. Nature Precedings. Full PDF Article
Luo, Y. et al. (Oct 2008). Abnormal DNA methylation in T cells from patients with subacute cutaneous lupus erythematosus. Br J Dermatol. 159(4): 827-33. PubMed Abstract
Liao, Yi-Jen et al. (Sep 9, 2008). Characterization of a glycine N-methyltransferase gene knockout mouse model for hepatocellular carcinoma: Implications of the gender disparity in liver cancer susceptibility. International Journal of Cancer Epub ahead of print. Abstract
Thaler, Roman et al. (Aug 2008). Epigenetic regulation of human buccal mucosa mitochondrial superoxide dismutase gene expression by diet. Br J Nutr. Epub ahead of print. PubMed Abstract
Suzuki, Maiko et al. (Mar 2008). Zebularine-induced reduction in VEGF secretion by HIF-1α degradation in oral squamous cell carcinoma. Molecular Medicine Reports 1: 465-471. Full PDF Article
Novikova, Svetlana I. et al. (Apr 2008). Maternal Cocaine Administration in Mice Alters DNA Methylation and Gene Expression in Hippocampal Neurons of Neonatal and Prepubertal Offspring. PLoS ONE 3(4): e1919. Full PDF Article
Liu, Chi et al. (Feb 2008). Plasma S-adenosylhomocysteine is a better biomarker of atherosclerosis than homocysteine in apolipoprotein E-deficient mice fed high dietary methionine. The Journal of Nutrition 138(2): 311-5. PubMed Abstract
Suzuki, Maiko et al. (December 2007). Epigenetic regulation of chemosensitivity to 5-fluorouracil and cisplatin by zebularine in oral squamous cell carcinoma. International Journal of Oncology. 31(6): 1449-56. Full PDF Article