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通用DNA甲基化定量试剂盒

【产品中文名称】

 通用DNA甲基化定量试剂盒

【产品产地】

 Epigentek

 English Name

 Methylamp Global DNA Methylation Quantification Kit

产品货号

 P-1014

 产品 价格/规格

 48/4624元,96/8624

 【产品详细说明】

    

Product Overview


The
Methylamp™ Global DNA Methylation Quantification Kit is a complete set of essential components which enables the experimenter to quantify global DNA methylation through an ELISA-like reaction using Epigentek's uniquely simplified and streamlined procedure. The entire procedure can be completed within only 4 hours in either manual or high throughput analysis. The Methylamp™ Global DNA Methylation Quantification Kit is suitable for detecting global DNA methylation status using genomic DNA isolated from variety of cultured cells, fresh and frozen tissue, paraffin-embedded tissue, plasma/serum sample, and body fluid sample, etc.

WHY CHOOSE THE METHYLAMP™ GLOBAL DNA METHYLATION QUANTIFICATION KIT?

  • Very rapid procedure, which can be finished within 4 hours.
  • Colorimetric quantification without radioactivity, extraction, and chromatography.
  • Strip microplate format makes the assay flexible: manual or high throughput analysis.
  • Simple, reliable, and consistent assay conditions.

Principle & Procedure


The Methylamp™ Global DNA Methylation Quantification Kit contains all reagents required for quantification of global DNA methylation. In this assay, DNA is immobilized to the strip well specifically treated to have high affinity to the DNA. The methylated fraction of DNA can be recognized by 5-methylcytosine antibody and quantified through an ELISA-like reaction. The amount of methylated DNA is proportional to the OD intensity.

SCHEMATIC PROCEDURE:




Quantification of Methylated DNA.

Product Components


GM1 (10x wash buffer)
GM2 (DNA binding solution)
GM3 (methylated DNA control,100 µg/ml)*
GM4 (block solution)
GM5 (capture antibody, 1 mg/ml)*
GM6 (detecting antibody, 100 µg/ml)*
GM7 (developing solution)
GM8 (stop solution)
8 well assay strips (with frame)
User guide

* Spin the solution down to the bottom before use.

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

[Material Safety Data Sheet]

References & Citations


Hervouet, Eric et al. (November 2008). Tumor induction by disruption of the Dnmt1, PCNA and UHRF1 interactions. Nature Precedings. Full PDF Article

Luo, Y. et al. (Oct 2008). Abnormal DNA methylation in T cells from patients with subacute cutaneous lupus erythematosus. Br J Dermatol.
159(4): 827-33. PubMed Abstract

Liao, Yi-Jen et al. (Sep 9, 2008). Characterization of a glycine N-methyltransferase gene knockout mouse model for hepatocellular carcinoma: Implications of the gender disparity in liver cancer susceptibility. International Journal of Cancer
Epub ahead of print. Abstract

Thaler, Roman et al. (Aug 2008). Epigenetic regulation of human buccal mucosa mitochondrial superoxide dismutase gene expression by diet. Br J Nutr.
Epub ahead of print. PubMed Abstract

Suzuki, Maiko et al. (Mar 2008). Zebularine-induced reduction in VEGF secretion by HIF-1α degradation in oral squamous cell carcinoma. Molecular Medicine Reports
1: 465-471. Full PDF Article

Novikova, Svetlana I. et al. (Apr 2008). Maternal Cocaine Administration in Mice Alters DNA Methylation and Gene Expression in Hippocampal Neurons of Neonatal and Prepubertal Offspring. PLoS ONE
3(4): e1919. Full PDF Article

Liu, Chi et al. (Feb 2008). Plasma S-adenosylhomocysteine is a better biomarker of atherosclerosis than homocysteine in apolipoprotein E-deficient mice fed high dietary methionine. The Journal of Nutrition
138(2): 311-5. PubMed Abstract

Suzuki, Maiko et al. (December 2007). Epigenetic regulation of chemosensitivity to 5-fluorouracil and cisplatin by zebularine in oral squamous cell carcinoma. International Journal of Oncology.
31(6): 1449-56. Full PDF Article


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