The Methylamp™ One-Step DNA Modification Kit is a complete set of essential components which enables the experimenter to perform DNA methylation analysis using Epigentek's uniquely simplified and streamlined bisulfite method. The entire procedure can be completed within a mere 1 hour and 45 minutes and produces far superior results than any competitor kits. The Methylamp™ One-Step DNA Modification Kit is suitable for MS-PCR, real time MS-PCR, methylation sequencing, and pyrosequencing, as well as methylation microarray.
WHY CHOOSE THE METHYLAMP™ ONE-STEP DNA MODIFICATION KIT?
- The number one fastest procedure available. The entire experiment can be finished within 1 hour and 45 minutes.
- Completely converts unmethylated cytosine into uracil: modified DNA > 99.98%.
- The lowest degradation of DNA in the modification process: more than 90% of DNA loss can be prevented.
- The lowest requirement of starting DNA for modification: only 50 pg or 20 cells.
- Extremely simple, reliable, and consistent modification conditions.
Principle & Procedure
The Methylamp™ One-Step DNA Modification Kit contains all reagents required for bisulfite conversion on a DNA sample. DNA is denatured by heating, which allows DNA denaturation and bisulfite modification to be carried out simultaneously. In the modification process, bisulfite reagent reacts specifically with single-stranded DNA, thereby deaminating cytosine and creating a uracil residue. The unique DNA protection reagents contained in the modification buffer can prevent the chemical and thermophilic degradation of DNA in the bisulfite treatment. The non-toxic modified DNA capture buffer enable DNA tightly to bind to the column filter, thus DNA cleaning can be carried out on the column to effectively remove residual sodium bisulfite and salts. Modified DNA then can be eluted and stably stored at –20°C for up to 2 months.
G1 (DNA modification powder)
G2 (DNA modification)
G3 (balance solution)
G4 (modified DNA capture)
G5 (modified DNA cleaning)
G6 (modified DNA elution)
User Guide & MSDS
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing firstname.lastname@example.org along with your contact information and institution name.
References & Citations
Cheetham, S. et al. (Jun 2008). SPARC promoter hypermethylation in colorectal cancers can be reversed by 5-Aza-2'deoxycytidine to increase SPARC expression and improve therapy response. British Journal of Cancer 98(11): 1810-9. PubMed Abstract
Otsubo, T. et al. (Jul 2007). SOX2 is frequently downregulated in gastric cancers and inhibits cell growth through cell-cycle arrest and apoptosis. British Journal of Cancer 98(4): 824-31. PubMed Abstract
Treilleux, I. et al. (Jul 2007). The molecular causes of low ATM protein expression in breast carcinoma; promoter methylation and levels of the catalytic subunit of DNA-dependent protein kinase. Histopathology 51(1): 63-9. PubMed Abstract